16S rRNA seq - healthy human feces - SRA

SRX1531758: 16S rRNA seq - healthy human feces
1 ILLUMINA (Illumina MiSeq) run: 375,086 spots, 225.2M bases, 135Mb downloads

Design: DNA was extracted from feces using QIAmp DNA Stool Mini Kit (Qiagen) according to the manufacturer’s instructions following 3 x 30s of homogenization using metal beads on a Retsch MixerMill MM 400 homogenizer. Metagenomic sequencing libraries were prepared according to the Illumina MiSeq system instructions. In brief, the V3 and V4 region of the 16S bacterial rRNA gene was amplified using recommended primers: Forward 5' TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG, and Reverse 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC, which create amplicons of ~460 bp. Amplicons were cleaned using AMPure XP beads step then adapters and dual-index barcodes (Nextera XT) were attached to the amplicons to facilitate multiplex sequencing.

Submitted by: University of British Columbia Okanagan

Study: Fecal bacterial diversity in humans

PRJNA308319 • SRP068480 • All experiments • All runs

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The aim of this study was to analyse the stool bacterial communities of humans with varying cardiorespiratory fitness levels to explore the relationship between physical fitness and gut bacterial diversity.

Sample:

SAMN04407446 • SRS1249080 • All experiments • All runs

Organism: human gut metagenome

Library:

Name: S11

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: SINGLE

Runs: 1 run, 375,086 spots, 225.2M bases, 135Mb

Run	# of Spots	# of Bases	Size	Published
SRR3102812	375,086	225.2M	135Mb	2016-01-19

ID:: 2168648

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